CRSEXP029

=Researcher= Matthew McBride =Objective= To determine the shift of D2O when using the coaxial NMR tube system with CDCl3 as the reference =Procedure= Add D2O to the inner coaxial NMR tube and CDCl3 to the outer NMR tube and obtain the HNMR spectrum. =Results= [|HNMR Spectrum] of D2O in the inner coaxial NMR tube with CDCl3 as the reference solvent in the outer tube: D2O has a peak at 4.778 ppm, CDCl3 at 7.259 ppm (appearing as a doublet), TMS peak at 0 ppm, a water peak likely from the CDCl3 at 1.54 ppm (multiplet), and an impurity at 2.17 ppm (doublet). =Discussion= The shift of D2O is [|reported] to be 4.8 ppm. The shift was observed to be at 4.778 ppm with D2O as the sample in the coaxial system and CDCl3 as the reference, in agreement with the reported D2O chemical shift. =Conclusions= D2O had the chemical shift of 4.778 ppm as the sample in the coaxial system and CDCl3 as the reference. =Log=

2013-10-15
16:00 Added D2O to the inner coaxial NMR tube (sample) and added CDCl3 to the outer coaxial NMR tube and obtained the HNMR spectrum using the 500 MHz NMR spectrometer.